Lipid Metabolism

(Voet & Voet Chapter 23)

1). How is lipid (triglycerides and phospholipids) digested, absorbed, and transported? What are the major enzymes involved?
2). Catabolism :
  a. How is the fatty acid (FA) activated (to what?) for oxidation? Where does it take place? How does it get the free energy for the activation?

b. Where does the ] oxidation of FA take place? How is the acyl CoA transported to this oxidation sites (via what kind of carrier)?

c. What is the relationship between FA ] oxidation and oxidative phosphorylation?

d.How is the odd chain FA oxidized? What are the major enzymes for the final C3 oxidation and what are the cofactors for these enzymes?

e. What is the significance of peroxisomal ] oxidation? What are the differences between peroxisomal and mitochondria FA oxidation?

f. How is the ketone body (what kind of compounds?) generated? What is its physiological significance?

3). Anabolism :
  a. What is the carrier for FA synthesis reaction? Acetyl CoA is converted to what intermediate for FA synthesis by which enzyme (and its cofactor)?

b. Where does FA synthesis take place? How is the acetyl CoA transported for the FA synthesis?

4). Which enzyme(s) is the key point for the regulation of FA metabolism? How?

5). Which enzyme is the key point for the regulation of cholesterol synthesis? Is it cAMP dependent? What is the key intermediate?

6). Arachidonate is synthesized from which essential FA? What kinds of biological active compounds are produced from arachidonate by cyclic and linear pathway, respectively?

7). What is the difference between glycerolipid and sphingolipid? How many kinds of sphingolipids are found in human body?

Practice Problem : Question 5 and 13, Chapter 23 (Voet & Voet, p.726)

Last modified on 1999.3.22 by K.-J. Hsiao (s)