Citric Acid Cycle

(Voet & Voet Chapter 16)

1). What kind of chemical bond between Acetyl group and CoA? What is the£GGo¡¦ for the hydrolysis of this bond, and compare to that of ATP hydrolysis.

2). TPP, Lipoic acid, and FAD reaction mechanism in the Pyruvate dehydrogenase (Pyr. DHase) enzyme complex.

3). How many components in the Pyr. DHase multienzyme complex? What is the advantage of a "multienzyme complex"?

4). How is the activity of Pyr. Dhase controlled? (NADH, acetyl-CoA, phosphorylation/dephosphorylation) Is it controlled by cAMP?

5). Stereochemistry of citrate (how could you/enzyme distinguish the two CH2COO- chains?)

6). How many high energy compounds are produced per acetyl group via the citric acid cycle.

7). What are the rate-controlling enzymes in the cycle and how is it controlled?

8). Which citric acid cycle intermediates can be used for what anabolic pathways?

9). Anaplerotic reaction and pyruvate carboxylase.

10). What citric acid cycle intermediates can be generated from which degradative pathways other than acetyl-CoA?

11). What are the other names for the ¡§citric acid cycle¡¨?


Practice Problem : Question 1 and 9 of Chapter 19 (Veot & Veot, p. 562)

Last modified on 1999.3.21 by K.-J. Hsiao (¿½¼s¤¯)                    [ ­º­¶ ]